Plasmid Vector - Detail
Request |
|
|
---|---|---|
NBRP ID | G90459 | |
Name | pTM1289 | |
Description | Multiplex CRISPR/Cas9 knockout system tRNA-sgRNA entry vector 4, pEV4 | |
Accession | ||
DSC-ID | ||
Associated Gene(s) | ||
Backbone Plasmid | p4HD from Platinum Gate TALEN Kit | |
Antibiotic Resistance | ampr neor | |
References |
Sekine R, Kawata T, Muramoto T.
CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium. Sci Rep (2018) 8(1) 8471 [PubMed ID = 29855514] [RRC reference] |
|
Map | Multiplex_CRISPR-Cas9_Assembly_System.pdf | |
Sequence | ||
Depositor | Tetsuya Muramoto | |
Position | ||
Source | Depositor | |
Other Information | Ideal for multiple gene knockouts See Fig. S3 for details of the vectors. Add AGCA to the 5′ end of the sense oligonucleotide and AAAC to the 5′ end of the antisense oligonucleotide for the Golden Gate reaction with BpiI. | |
Use Condition | B1 |