Strain - Detail

NBRP ID S00008
Strain Name gefT-
Strain Summary knockout of candidate cGMP-binding protein GbpC containing RasGEF domain; blasticidin resistant
Strain Descriptor gbpC-
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain DH1
Mutagenesis Method homologous recombination
Mutagenesis Detail pcr pruducts with selection cassette
Associated Gene gbpC 
Foreign Gene bsR
Genotype axeA1, axeB1, axeC1, bsR,gbpC-[gbpC-bsr],pyr5-6-[pRG24],ura-
Phenotype axenic, blasticidin resistant, uracil auxotroph
Axenic Growth Y
Viable Spore Formation
References Goldberg JM, Bosgraaf L, Van Haastert PJ, Smith JL.
Identification of four candidate cGMP targets in Dictyostelium.
Proc Natl Acad Sci U S A  (2002)  99(10)   6749-54  
[PubMed ID = 12011437]  [RRC reference
Bosgraaf L, Russcher H, Smith JL, Wessels D, Soll DR, Van Haastert PJ.
A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium.
EMBO J  (2002)  21(17)   4560-70  
[PubMed ID = 12198158]  [RRC reference
Bosgraaf L, Waijer A, Engel R, Visser AJ, Wessels D, Soll D, van Haastert PJ.
RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium.
J Cell Sci  (2005)  118(Pt 9)   1899-910  
[PubMed ID = 15827084]  [RRC reference
Other Information originally deposited by Peter van Haastert to Dicty Stock Center
Depositor DSC
Source P. Haastert
Use Condition A
DSC DBS0235995