Strain - Detail

NBRP ID S00142
Strain Name pde5-, pdeD-
Strain Summary gbpA null mutant
Strain Descriptor gbpA-
Systematic Name DBS0235991
Species Dictyostelium discoideum
Category Recombinant
Parental Strain DH1
Mutagenesis Method homologous recombination
Mutagenesis Detail pcr pruducts with selection cassette
Associated Gene pdeD 
Foreign Gene bsR
Genotype axeA1, axeB1, axeC1, bsR,pyr5-6-[pRG24],pdeD-[gbpA-bsr]
Phenotype axenic,blasticidin resistant, null mutant
Axenic Growth Y
Viable Spore Formation OK
References Goldberg JM, Bosgraaf L, Van Haastert PJ, Smith JL.
Identification of four candidate cGMP targets in Dictyostelium.
Proc Natl Acad Sci U S A  (2002)  99(10)   6749-54  
[PubMed ID = 12011437]  [RRC reference
Bosgraaf L, Russcher H, Smith JL, Wessels D, Soll DR, Van Haastert PJ.
A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium.
EMBO J  (2002)  21(17)   4560-70  
[PubMed ID = 12198158]  [RRC reference
Bosgraaf L, Russcher H, Snippe H, Bader S, Wind J, Van Haastert PJ.
Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium.
Mol Biol Cell  (2002)  13(11)   3878-89  
[PubMed ID = 12429832]  [RRC reference
Other Information
Depositor DSC
Source P. Haastert
Use Condition A
DSC DBS0235991