Strain - Detail

NBRP ID S00143
Strain Name pde6-, pdeE-
Strain Summary gbpB null mutant
Strain Descriptor gbpB-
Systematic Name DBS0235993
Species Dictyostelium discoideum
Category Recombinant
Parental Strain DH1
Mutagenesis Method homologous recombination
Mutagenesis Detail pcr pruducts with selection cassette
Associated Gene pdeE 
Foreign Gene bsR
Genotype axeA1, axeB1, axeC1, pyr5-6-[pRG24],pdeE--[gbpB-pyr],ura+
Phenotype axenic, null mutant
Axenic Growth Y
Viable Spore Formation OK
References Kuwayama H, Kubohara Y.
Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxis.
PLoS One  (2009)  4(8)   e6658  
[PubMed ID = 19684855]  [RRC reference
Goldberg JM, Bosgraaf L, Van Haastert PJ, Smith JL.
Identification of four candidate cGMP targets in Dictyostelium.
Proc Natl Acad Sci U S A  (2002)  99(10)   6749-54  
[PubMed ID = 12011437]  [RRC reference
Bosgraaf L, Russcher H, Smith JL, Wessels D, Soll DR, Van Haastert PJ.
A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium.
EMBO J  (2002)  21(17)   4560-70  
[PubMed ID = 12198158]  [RRC reference
Bosgraaf L, Waijer A, Engel R, Visser AJ, Wessels D, Soll D, van Haastert PJ.
RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium.
J Cell Sci  (2005)  118(Pt 9)   1899-910  
[PubMed ID = 15827084]  [RRC reference
Kubohara Y, Kikuchi H, Nguyen VH, Kuwayama H, Oshima Y.
Evidence that differentiation-inducing factor-1 controls chemotaxis and cell differentiation, at least in part, via mitochondria in <i>D. discoideum</i>.
Biol Open  (2017)  6(6)   741-751  
[PubMed ID = 28619991]  [RRC reference
Other Information
Depositor DSC
Source P. Haastert
Use Condition A
DSC DBS0235993