Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00244 | |
| Strain Name | FCL-AA17(DdFHa-) | |
| Strain Summary | DdFHa null mutant; axenic; resistant to blasticidin; axenic | |
| Strain Descriptor | ||
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | KAX3 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | the 5' (1530 nt) fragment of DdFHa cDNA was excised by digestion of FCLAA17 with Kpn I-Sma I, and then inserted into the Bam HI site of pBsrĪBam (Adachi et al., 1994) in the sense orientation. The 3' (531-1313 nt) fragment was excised by Kpn I-Bam HI dig | |
| Associated Gene | DdFHa fhbA | |
| Foreign Gene | bsR | |
| Genotype | ||
| Phenotype | ||
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Iijima M, Shimizu H, Tanaka Y, Urushihara H.
Identification and characterization of two flavohemoglobin genes in Dictyostelium discoideum. Cell Struct Funct (2000) 25(1) 47-55 [PubMed ID = 10791894] [RRC reference] Hirose S, Hesnard J, Ghazi N, Roussel D, Voituron Y, Cochet-Escartin O, Rieu JP, Anjard C, Funamoto K. The aerotaxis of <i>Dictyostelium discoideum</i> is independent of mitochondria, nitric oxide and oxidative stress. Front Cell Dev Biol (2023) 11 1134011 [PubMed ID = 37397260] [RRC reference] |
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| Other Information | ||
| Depositor | Hideko Urushihara | |
| Position | ||
| Source | depositor | |
| Use Condition | B1 | |
| DSC | ||
| Remarks | ||
| Images | ||
| Sequence | ||
