Strain - Detail

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NBRP ID S00249
Strain Name rasD-
Strain Summary rasD null mutant in AX2; resistant to blasticidin; axenic
Strain Descriptor
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain Ax2
Mutagenesis Method homologous recombination
Mutagenesis Detail pATW5
Associated Gene rasD 
Foreign Gene bsR
Genotype axeA2, axeB2, axeC2, rasD-[pATW5], bsR
Phenotype
Axenic Growth Y
Viable Spore Formation OK
References Wilkins A, Khosla M, Fraser DJ, Spiegelman GB, Fisher PR, Weeks G, Insall RH.
Dictyostelium RasD is required for normal phototaxis, but not differentiation.
Genes Dev  (2000)  14(11)   1407-13  
[PubMed ID = 10837033]  [RRC reference
Other Information
Depositor Hideko Urushihara
Source depositor
Use Condition B1
DSC
Remarks
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Sequence
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