Strain - Detail

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NBRP ID S00296
Strain Name RacF2-CA01(KAX3)
Strain Summary overexpressor of constitutively-active RacF2 in KAX3; resistant to neomycin; axenic
Strain Descriptor KAX3/[act15]:gfp:racF2(G12V)
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain KAX3
Mutagenesis Method random insertion
Mutagenesis Detail pGFP:RacF2(G12V)
Associated Gene racF2 
Foreign Gene neor, gfp
Genotype axeA1, axeB1, axeC1, [pRacF2-CA], neoR, gfp
Phenotype
Axenic Growth Y
Viable Spore Formation OK
References Muramoto T, Urushihara H.
Small GTPase RacF2 affects sexual cell fusion and asexual development in Dictyostelium discoideum through the regulation of cell adhesion.
Dev. Growth Differ.  (2006)  48(3)   199-208  
[PubMed ID = 16573737]  [RRC reference
Other Information racF2=GEG02
Depositor Hideko Urushihara
Source T. Muramoto
Use Condition A
DSC
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