Strain - Detail

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NBRP ID S00333
Strain Name p34-arc mutant
Strain Summary Randomly mutagenized arcB with 10 mutations (arcB1 allele).
Strain Descriptor arcB(I191L/D197Y/K206V/A213V/F223L/P224S/ E232G/I237T/H245L/S250S)
Systematic Name HM2245
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX2 (DBS0235521)
Mutagenesis Method homologous recombination
Mutagenesis Detail endogenous substitution, pPL41
Associated Gene arcB 
Foreign Gene bsR
Genotype axeA2, axeB2, axeC2, arcB1[pPL41], bsR
Phenotype axenic, mutant, knock-in
Axenic Growth Y
Viable Spore Formation OK
References Langridge PD, Kay RR.
Mutants in the Dictyostelium Arp2/3 complex and chemoattractant-induced actin polymerization.
Exp. Cell Res.  (2007)  313(12)   2563-74  
[PubMed ID = 17553489]  [RRC reference
Other Information
Depositor DSC
Source R. Kay
Use Condition A
DSC DBS0238411
Remarks
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Sequence
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