Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00334 | |
| Strain Name | KP, pkaC overexpressor, DdPK2 overexpressor, K-P | |
| Strain Summary | pkaC overexpressor, regulated by its endogenous upstream region | |
| Strain Descriptor | [pkaC]:pkaC | |
| Systematic Name | DBS0236484 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX2 | |
| Mutagenesis Method | random insertion | |
| Mutagenesis Detail | exogenous mutation, K-Neo | |
| Associated Gene | pkaC | |
| Foreign Gene | neoR | |
| Genotype | axeA2,axeB2,axeC2,[K-Neo],neoR | |
| Phenotype | neomycin resistant, overexpressor, axenic, mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Anjard C, Pinaud S, Kay RR, Reymond CD.
Overexpression of Dd PK2 protein kinase causes rapid development and affects the intracellular cAMP pathway of Dictyostelium discoideum. Development (1992) 115(3) 785-90 [PubMed ID = 1330484] [RRC reference] Anjard C, Chang WT, Gross J, Nellen W. Production and activity of spore differentiation factors (SDFs) in Dictyostelium. Development (1998) 125(20) 4067-75 [PubMed ID = 9735367] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | P. Newell | |
| Use Condition | A | |
| DSC | DBS0236484 | |
| Remarks | ||
| Images | ||
| Sequence | ||
