Strain - Detail

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NBRP ID S00334
Strain Name KP, pkaC overexpressor, DdPK2 overexpressor, K-P
Strain Summary pkaC overexpressor, regulated by its endogenous upstream region
Strain Descriptor [pkaC]:pkaC
Systematic Name DBS0236484
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX2
Mutagenesis Method random insertion
Mutagenesis Detail exogenous mutation, K-Neo
Associated Gene pkaC 
Foreign Gene neoR
Genotype axeA2,axeB2,axeC2,[K-Neo],neoR
Phenotype neomycin resistant, overexpressor, axenic, mutant
Axenic Growth Y
Viable Spore Formation OK
References Anjard C, Pinaud S, Kay RR, Reymond CD.
Overexpression of Dd PK2 protein kinase causes rapid development and affects the intracellular cAMP pathway of Dictyostelium discoideum.
Development  (1992)  115(3)   785-90  
[PubMed ID = 1330484]  [RRC reference
Anjard C, Chang WT, Gross J, Nellen W.
Production and activity of spore differentiation factors (SDFs) in Dictyostelium.
Development  (1998)  125(20)   4067-75  
[PubMed ID = 9735367]  [RRC reference
Other Information
Depositor DSC
Source P. Newell
Use Condition A
DSC DBS0236484
Remarks
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