Strain - Detail

NBRP ID S00335
Strain Name Akt/PKB null, PKB-
Strain Summary pkbA null mutant
Strain Descriptor pkbA-
Systematic Name DBS0236784
Species Dictyostelium discoideum
Category Recombinant
Parental Strain KAX3
Mutagenesis Method homologous recombination
Mutagenesis Detail pkb-bsR (bsR cassette at position 520 of Akt/PKB ORF)
Associated Gene pkbA 
Foreign Gene bsR
Genotype axeA1,axeB1,axeC1,pkbA-[pkb-bsR],bsR
Phenotype axenic,blasticidin resistant, null mutant
Axenic Growth Y
Viable Spore Formation
References Chung CY, Potikyan G, Firtel RA.
Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKa.
Mol Cell  (2001)  7(5)   937-47  
[PubMed ID = 11389841]  [RRC reference
Meili R, Ellsworth C, Lee S, Reddy TB, Ma H, Firtel RA.
Chemoattractant-mediated transient activation and membrane localization of Akt/PKB is required for efficient chemotaxis to cAMP in Dictyostelium.
EMBO J  (1999)  18(8)   2092-105  
[PubMed ID = 10205164]  [RRC reference
Other Information
Depositor Satoshi Sawai
Source DSC
Use Condition A
DSC DBS0236784