Strain - Detail

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NBRP ID S00337
Strain Name gc-1, gc-2, gca-/sgc-
Strain Summary gca/sgcA double null mutant in DH1(guanylyl cyclases GCA and sGC)
Strain Descriptor gca-/sgcA-
Systematic Name DBS0236000
Species Dictyostelium discoideum
Category Recombinant
Parental Strain DH1
Mutagenesis Method homologous recombination
Mutagenesis Detail ddgca-bsr„ÄĀpsCycKOpyr
Associated Gene gcA  sgcA 
Foreign Gene bsR
Genotype axeA1, axeB1, axeC1, pyr5-6-[pRG24], sgcA-[psCycKOpyr],ura+,gca-[ddgca-bsr]
Phenotype axenic,blasticidin resistant, multiple null mutant
Axenic Growth Y
Viable Spore Formation OK
References Roelofs J, Snippe H, Kleineidam RG, Van Haastert PJ.
Guanylate cyclase in Dictyostelium discoideum with the topology of mammalian adenylate cyclase.
Biochem. J.  (2001)  354(Pt 3)   697-706  
[PubMed ID = 11237875]  [RRC reference
Roelofs J, Meima M, Schaap P, Van Haastert PJ.
The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase.
EMBO J.  (2001)  20(16)   4341-8  
[PubMed ID = 11500361]  [RRC reference
Roelofs J, Van Haastert PJ.
Characterization of two unusual guanylyl cyclases from dictyostelium.
J. Biol. Chem.  (2002)  277(11)   9167-74  
[PubMed ID = 11777934]  [RRC reference
Other Information
Depositor Satoshi Sawai
Source DSC
Use Condition A
DSC DBS0236000
Remarks
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