Strain - Detail

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NBRP ID S00338
Strain Name pde6-, pdeE-
Strain Summary gbpA null mutant
Strain Descriptor gbpB-
Systematic Name DBS0235993
Species Dictyostelium discoideum
Category Recombinant
Parental Strain DH1
Mutagenesis Method homologous recombination
Mutagenesis Detail pcr pruducts with selection cassettes
Associated Gene pdeE 
Foreign Gene
Genotype axeA1, axeB1, axeC1, pyr5-6-[pRG24],pdeE--[gbpB-pyr],ura+
Phenotype axenic, null mutant
Axenic Growth Y
Viable Spore Formation OK
References Kuwayama H, Kubohara Y.
Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxis.
PLoS ONE  (2009)  4(8)   e6658  
[PubMed ID = 19684855]  [RRC reference
Goldberg JM, Bosgraaf L, Van Haastert PJ, Smith JL.
Identification of four candidate cGMP targets in Dictyostelium.
Proc. Natl. Acad. Sci. U.S.A.  (2002)  99(10)   6749-54  
[PubMed ID = 12011437]  [RRC reference
Bosgraaf L, Russcher H, Smith JL, Wessels D, Soll DR, Van Haastert PJ.
A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium.
EMBO J.  (2002)  21(17)   4560-70  
[PubMed ID = 12198158]  [RRC reference
Bosgraaf L, Waijer A, Engel R, Visser AJ, Wessels D, Soll D, van Haastert PJ.
RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium.
J. Cell. Sci.  (2005)  118(Pt 9)   1899-910  
[PubMed ID = 15827084]  [RRC reference
Other Information
Depositor Satoshi Sawai
Source DSC
Use Condition A
DSC DBS0235993
Remarks
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