Strain - Detail

NBRP ID S00339
Strain Name gbpA-/gbpB-
Strain Summary gbpA/gbpB double null mutant
Strain Descriptor gbpA-/gbpB-
Systematic Name DBS0235992
Species Dictyostelium discoideum
Category Recombinant
Parental Strain DH1
Mutagenesis Method homologous recombination
Mutagenesis Detail pcr pruducts with selection cassettes
Associated Gene pdeD  pdeE 
Foreign Gene bsR
Genotype axeA1, axeB1, axeC1,bsR,pdeD-[gbpA-bsr],pdeE-[gbpB-pyr], pyr5-6-[pRG24],ura+
Phenotype axenic,blasticidin resistant, multiple null mutant
Axenic Growth Y
Viable Spore Formation OK
References Goldberg JM, Bosgraaf L, Van Haastert PJ, Smith JL.
Identification of four candidate cGMP targets in Dictyostelium.
Proc Natl Acad Sci U S A  (2002)  99(10)   6749-54  
[PubMed ID = 12011437]  [RRC reference
Bosgraaf L, Russcher H, Smith JL, Wessels D, Soll DR, Van Haastert PJ.
A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium.
EMBO J  (2002)  21(17)   4560-70  
[PubMed ID = 12198158]  [RRC reference
Bosgraaf L, Russcher H, Snippe H, Bader S, Wind J, Van Haastert PJ.
Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium.
Mol Biol Cell  (2002)  13(11)   3878-89  
[PubMed ID = 12429832]  [RRC reference
Other Information
Depositor Satoshi Sawai
Source DSC
Use Condition A
DSC DBS0235992