Strain - Detail

NBRP ID S00342
Strain Name rasC-
Strain Summary rasC null in AX2
Strain Descriptor rasC-/AX2
Systematic Name DBS0236853
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX2 (GW)
Mutagenesis Method homologous recombination
Mutagenesis Detail rasC::bsr
Associated Gene rasC 
Foreign Gene bsR
Genotype axeA2, axeB2, axeC2, bsR, rasC-[rasC::bsr]
Phenotype axenic, blasticidin resistant, drug resistant, null mutant
Axenic Growth Y
Viable Spore Formation NG
References Lim CJ, Spiegelman GB, Weeks G.
RasC is required for optimal activation of adenylyl cyclase and Akt/PKB during aggregation.
EMBO J  (2001)  20(16)   4490-9  
[PubMed ID = 11500376]  [RRC reference
Wessels D, Brincks R, Kuhl S, Stepanovic V, Daniels KJ, Weeks G, Lim CJ, Spiegelman G, Fuller D, Iranfar N, Loomis WF, Soll DR.
RasC plays a role in transduction of temporal gradient information in the cyclic-AMP wave of Dictyostelium discoideum.
Eukaryot Cell  (2004)  3(3)   646-62  
[PubMed ID = 15189986]  [RRC reference
Kamimura Y, Miyanaga Y, Ueda M.
Heterotrimeric G-protein shuttling via Gip1 extends the dynamic range of eukaryotic chemotaxis.
Proc Natl Acad Sci U S A  (2016)  113(16)   4356-61  
[PubMed ID = 27044073]  [RRC reference
Other Information
Depositor Satoshi Sawai
Source DSC
Use Condition A
DSC DBS0236853