Strain - Detail

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NBRP ID S00343
Strain Name DH1
Strain Summary Peter Devreotes original lab strain; axenic; uracil auxotroph
Strain Descriptor DH1
Systematic Name DBS0235696
Species Dictyostelium discoideum
Category Other
Parental Strain AX3 (DBS0235542)
Mutagenesis Method homologous recombination
Mutagenesis Detail endogenous deletion, pRG24 + FOA selection
Associated Gene pyr56 
Foreign Gene bsR
Genotype axeA1,axeB1,axeC1,pyr5-6-[pRG24],ura-,cmfA-[pCMF-KO],bsR
Phenotype uracil auxotroph, axenic
Axenic Growth Y
Viable Spore Formation OK
References Caterina MJ, Milne JL, Devreotes PN.
Mutation of the third intracellular loop of the cAMP receptor, cAR1, of Dictyostelium yields mutants impaired in multiple signaling pathways.
J Biol Chem  (1994)  269(2)   1523-32  
[PubMed ID = 8288619]  [RRC reference
Other Information
Depositor DSC
Position
Source P.Devreotes
Use Condition A
DSC DBS0235696
Remarks
Images
Sequence
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