Strain - Detail

NBRP ID S00406
Strain Name PI3K1-5-, PTEN- sextuple mutant, PI3K1-5-/PTEN- sextuple mutant, pikA2-/pikB2-/pikC2-/pikF2-/pikG2-/pteN1-
Strain Summary pikA2-/pikB2-/pikC2-/pikF2-/pikG2-/pten1- sextuple mutant (allele number 1 denotes bsR cassette present; allele number 2 denotes removal of the bsR cassette, but still leaving one lox site).
Strain Descriptor pikA-/pikB-/pikC-/pikF-/pikG-/pten-
Systematic Name HM1295
Species Dictyostelium discoideum
Category Recombinant
Parental Strain pikA-/pikB-/pikC/pikF-/pikG- (DBS0238246)
Mutagenesis Method homologous recombination
Mutagenesis Detail pOH47, pOH74,pOH89, pOH88,pOH109R, pOH207
Associated Gene pikA  pikB  pikF  pikC  pikG  pten 
Foreign Gene bsR
Genotype axeA2, axeB2, axeC2, pikA2-[pOH74], pikB2-[pOH47], pikC2-[pOH109R], pikF2-[pOH89], pikG2-[pOH88], pten1-[pOH207], bsR
Phenotype axenic, blasticidin resistant, multiple null mutant,
Axenic Growth Y
Viable Spore Formation OK (poor)
References Hoeller O, Kay RR.
Chemotaxis in the absence of PIP3 gradients.
Curr Biol  (2007)  17(9)   813-7  
[PubMed ID = 17462897]  [RRC reference
Peracino B, Balest A, Bozzaro S.
Phosphoinositides differentially regulate bacterial uptake and Nramp1-induced resistance to Legionella infection in Dictyostelium.
J Cell Sci  (2010)  123(Pt 23)   4039-51  
[PubMed ID = 21045112]  [RRC reference
Other Information
Depositor DSC
Source R. Kay
Use Condition A
DSC DBS0252654