Strain - Detail

NBRP ID S00408
Strain Name sgkAOE, sgkA-OE
Strain Summary sgkA overexpressor, FLAG tagged
Strain Descriptor [act15]:sgkA:FLAG
Systematic Name DBS0237193, SA604
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX3-ORF+
Mutagenesis Method extrachromosomal plasmid
Mutagenesis Detail exogenous mutation, pDXA3C-sgkA-FLAG
Associated Gene sgkA 
Foreign Gene neoR, FLAG
Genotype axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6],ura+, [act15/sgkA:FLAG], neoR
Phenotype axenic, FLAG-tagged, mutant,neomycin resistant, overexpressor,
Axenic Growth Y
Viable Spore Formation OK
References Min J, Traynor D, Stegner AL, Zhang L, Hanigan MH, Alexander H, Alexander S.
Sphingosine kinase regulates the sensitivity of Dictyostelium discoideum cells to the anticancer drug cisplatin.
Eukaryot Cell  (2005)  4(1)   178-89  
[PubMed ID = 15643073]  [RRC reference
Other Information neomycinG418 10ug/ml
Depositor DSC
Source S. Alexander
Use Condition A
DSC DBS0237193