Strain - Detail

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NBRP ID S00412
Strain Name DS211C, severin-null
Strain Summary a truncated version of severin (domains 2+3). See pmid 1591239,Severin knockout obtained by transformation of AX2 with DS211C,
Strain Descriptor sevA-
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX2
Mutagenesis Method homologous recombination
Mutagenesis Detail endogenous insertion, pDNeo2 -DS211C
Associated Gene sevA 
Foreign Gene neoR
Genotype axeA2, axeB2, axeC2, sevA-[DS211C], neoR
Phenotype axenic,null mutant, neomycin resistant
Axenic Growth Y
Viable Spore Formation OK
References Eichinger L, Schleicher M.
Characterization of actin- and lipid-binding domains in severin, a Ca(2+)-dependent F-actin fragmenting protein.
Biochemistry  (1992)  31(20)   4779-87  
[PubMed ID = 1591239]  [RRC reference
Fisher PR, Noegel AA, Fechheimer M, Rivero F, Prassler J, Gerisch G.
Photosensory and thermosensory responses in Dictyostelium slugs are specifically impaired by absence of the F-actin cross-linking gelation factor (ABP-120).
Curr. Biol.  (1997)  7(11)   889-92  
[PubMed ID = 9480045]  [RRC reference
Other Information
Depositor DSC
Source P.Fisher
Use Condition A
DSC DBS0235923
Remarks
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