Strain - Detail

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NBRP ID S00416
Strain Name abpA-/abpC-/sevA-,HG1397
Strain Summary abpA-/abpC-/sevA- triple null mutant (alpha-actinin, 120 kDa gelation factor, severin) produced by a combination of chemical mutagenesis, homologous recombination, and parasexual genetics.
Strain Descriptor abpA-/abpC-/sevA-
Systematic Name HG1397
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX2
Mutagenesis Method homologous recombination
Mutagenesis Detail
Associated Gene abpA  abpC  sevA 
Foreign Gene neoR
Genotype axeA2, axeB2, axeC2,abp-tr(3), gelA1000, neoR, sevA1000
Phenotype axenic,drug resistant, neomycin resistant, multiple null mutant
Axenic Growth Y
Viable Spore Formation NO
References Witke W, Schleicher M, Noegel AA.
Redundancy in the microfilament system: abnormal development of Dictyostelium cells lacking two F-actin cross-linking proteins.
Cell  (1992)  68(1)   53-62  
[PubMed ID = 1732064]  [RRC reference
Witke W, Nellen W, Noegel A.
Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein.
EMBO J.  (1987)  6(13)   4143-8  
[PubMed ID = 2832153]  [RRC reference
Schindl M, Wallraff E, Deubzer B, Witke W, Gerisch G, Sackmann E.
Cell-substrate interactions and locomotion of Dictyostelium wild-type and mutants defective in three cytoskeletal proteins: a study using quantitative reflection interference contrast microscopy.
Biophys. J.  (1995)  68(3)   1177-90  
[PubMed ID = 7756537]  [RRC reference
Other Information
Depositor DSC
Source G.Gerisch
Use Condition A
DSC DBS0236169