Strain - Detail
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NBRP ID | S00442 | |
Strain Name | GFP-3Ala/HS1 | |
Strain Summary | HS1 mhcA-null cells expressing GFP-mhcA with the 3 Ala mutation | |
Strain Descriptor | mhcA-/[act15]:GFP-mhcA (3 Ala) | |
Systematic Name | ||
Species | Dictyostelium discoideum | |
Category | Recombinant | |
Parental Strain | HS1 | |
Mutagenesis Method | extrachromosomal_plasmid | |
Mutagenesis Detail | pTIKL GFP 3 Ala myo | |
Associated Gene | mhcA | |
Foreign Gene | neoR, gfp | |
Genotype | axeA1, axeB1, axeC1, neoR | |
Phenotype | axenic, G418 resistant | |
Axenic Growth | Y | |
Viable Spore Formation | OK (poor) | |
References |
Egelhoff TT, Brown SS, Spudich JA.
Spatial and temporal control of nonmuscle myosin localization: identification of a domain that is necessary for myosin filament disassembly in vivo. J Cell Biol (1991) 112(4) 677-88 [PubMed ID = 1899668] [RRC reference] Sabry JH, Moores SL, Ryan S, Zang JH, Spudich JA. Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesis in live cells. Mol Biol Cell (1997) 8(12) 2605-15 [PubMed ID = 9398679] [RRC reference] |
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Other Information | ||
Depositor | Taro Uyeda | |
Position | ||
Source | depositor | |
Use Condition | A | |
DSC | ||
Remarks | ||
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Sequence |