Strain - Detail

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NBRP ID S00442
Strain Name GFP-3Ala/HS1
Strain Summary HS1 mhcA-null cells expressing GFP-mhcA with the 3 Ala mutation
Strain Descriptor mhcA-/[act15]:GFP-mhcA (3 Ala)
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain HS1
Mutagenesis Method extrachromosomal_plasmid
Mutagenesis Detail pTIKL GFP 3 Ala myo
Associated Gene mhcA 
Foreign Gene neoR, gfp
Genotype axeA1, axeB1, axeC1, neoR
Phenotype axenic, G418 resistant
Axenic Growth Y
Viable Spore Formation OK (poor)
References Egelhoff TT, Brown SS, Spudich JA.
Spatial and temporal control of nonmuscle myosin localization: identification of a domain that is necessary for myosin filament disassembly in vivo.
J. Cell Biol.  (1991)  112(4)   677-88  
[PubMed ID = 1899668]  [RRC reference
Sabry JH, Moores SL, Ryan S, Zang JH, Spudich JA.
Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesis in live cells.
Mol. Biol. Cell  (1997)  8(12)   2605-15  
[PubMed ID = 9398679]  [RRC reference
Other Information
Depositor Taro Uyeda
Source depositor
Use Condition A
DSC
Remarks
Images
Sequence
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