Strain - Detail

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NBRP ID S00447
Strain Name atg5-
Strain Summary atg5 null mutant in DH1
Strain Descriptor atg5-
Systematic Name HR75
Species Dictyostelium discoideum
Category Recombinant
Parental Strain DH1
Mutagenesis Method homologous recombination
Mutagenesis Detail atg5-KO (apg5-KO),endogenous mutation
Associated Gene atg5 
Foreign Gene bsR
Genotype axeA1,axeB1,axeC1,bsR,pyr5-6-[pRG24],ura-,atg5-[atg5-KO],
Phenotype axenic,, auxotroph, blasticidin resistant, null mutant
Axenic Growth Y
Viable Spore Formation NO
References Otto GP, Wu MY, Kazgan N, Anderson OR, Kessin RH.
Macroautophagy is required for multicellular development of the social amoeba Dictyostelium discoideum.
J Biol Chem  (2003)  278(20)   17636-45  
[PubMed ID = 12626495]  [RRC reference
Otto GP, Wu MY, Clarke M, Lu H, Anderson OR, Hilbi H, Shuman HA, Kessin RH.
Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideum.
Mol Microbiol  (2004)  51(1)   63-72  
[PubMed ID = 14651611]  [RRC reference
Other Information
Depositor DSC
Source M. Wu (G. Otto)
Use Condition A
DSC DBS0236371
Remarks
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