Strain - Detail

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NBRP ID S00449
Strain Name atg7-
Strain Summary atg7 null mutant in DH1
Strain Descriptor atg7-
Systematic Name HR79
Species Dictyostelium discoideum
Category Recombinant
Parental Strain DH1
Mutagenesis Method homologous Recombination
Mutagenesis Detail atg7-KO (apg7-KO),endogenous mutation
Associated Gene atg7 
Foreign Gene bsR
Genotype atg7-[atg7-KO],axeA1,axeB1,axeC1,ura-,vpyr5-6-[pRG24]
Phenotype auxotroph, axenic,,blasticidin resistant, null mutant,
Axenic Growth Y
Viable Spore Formation NO
References Otto GP, Wu MY, Kazgan N, Anderson OR, Kessin RH.
Macroautophagy is required for multicellular development of the social amoeba Dictyostelium discoideum.
J Biol Chem  (2003)  278(20)   17636-45  
[PubMed ID = 12626495]  [RRC reference
Otto GP, Wu MY, Clarke M, Lu H, Anderson OR, Hilbi H, Shuman HA, Kessin RH.
Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideum.
Mol Microbiol  (2004)  51(1)   63-72  
[PubMed ID = 14651611]  [RRC reference
Other Information
Depositor DSC
Source M.Wu (G.Otto)
Use Condition A
DSC DBS0236372
Remarks
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