Notice: Suspension of Services Due to the Network maintenance work of NIG

The services on our website will be stopped during the period of time stated below due to the network maintenance work of NIG. We apologize for the inconvenience and we thank you for your understanding in this matter.

Sat. 6th Jun. 2020 8:30 -17:30

Strain - Detail

NBRP ID S00454
Strain Name TL141
Strain Summary csaA- (gp80) knockout in lagC- mutant (gp150). Similar to TL122.
Strain Descriptor csaA-/lagC-
Systematic Name TL141
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AK127
Mutagenesis Method homologous recombination
Mutagenesis Detail csaA-KO
Associated Gene tgrC1  csaA 
Foreign Gene bsR
Genotype axeA1, axeB1, axeC1, pyr5-6-[pΔPYR-BglII], ura+, lagC-[DIV2],csaA-[csaA-KO],bsR
Phenotype blasticidin resistant, axenic, multiple null mutant
Axenic Growth Y
Viable Spore Formation OK
References Dynes JL, Clark AM, Shaulsky G, Kuspa A, Loomis WF, Firtel RA.
LagC is required for cell-cell interactions that are essential for cell-type differentiation in Dictyostelium.
Genes Dev.  (1994)  8(8)   948-58  
[PubMed ID = 7926779]  [RRC reference
Wang J, Hou L, Awrey D, Loomis WF, Firtel RA, Siu CH.
The membrane glycoprotein gp150 is encoded by the lagC gene and mediates cell-cell adhesion by heterophilic binding during Dictyostelium development.
Dev. Biol.  (2000)  227(2)   734-45  
[PubMed ID = 11071787]  [RRC reference
Other Information
Depositor DSC
Source B. Loomis
Use Condition A
DSC DBS0236989