Strain - Detail

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NBRP ID S00464
Strain Name GFP-L596S S1/pten-
Strain Summary pten-null cells expressing GFP-L596S mhcA S1
Strain Descriptor GFP-L596SS1/pten-
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain pten- (DBS0236830)
Mutagenesis Method extrachromosomal_plasmid
Mutagenesis Detail
Associated Gene pten  mhcA 
Foreign Gene bsR, neoR, gfp
Genotype axeA2,axeB2,axeC2,ptnA-[PTEN-BSR],bsR,neoR
Phenotype axenic, G418 resistant, blasticidin resistant, overexpressor
Axenic Growth Y
Viable Spore Formation no
References Uyeda TQ, Iwadate Y, Umeki N, Nagasaki A, Yumura S.
Stretching actin filaments within cells enhances their affinity for the myosin II motor domain.
PLoS ONE  (2011)  6(10)   e26200  
[PubMed ID = 22022566]  [RRC reference
Other Information
Depositor Taro Uyeda
Source depositor
Use Condition A
DSC
Remarks
Images
Sequence
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