Strain - Detail

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NBRP ID S00465
Strain Name GFP-S332D/G607A myoBS1dC/AX2
Strain Summary AX2 cells expressing GFP-motor domain of myoB carrying S332D/G607A mutations
Strain Descriptor GFP-S332DG607AmyoBS1dC/AX2
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX2
Mutagenesis Method extrachromosomal_plasmid
Mutagenesis Detail
Associated Gene myoB 
Foreign Gene neoR, gfp
Genotype axeA2,axeB2,axeC2,neoR
Phenotype axenic, G418 resistant, overexpressor
Axenic Growth Y
Viable Spore Formation OK
References Uyeda TQ, Iwadate Y, Umeki N, Nagasaki A, Yumura S.
Stretching actin filaments within cells enhances their affinity for the myosin II motor domain.
PLoS ONE  (2011)  6(10)   e26200  
[PubMed ID = 22022566]  [RRC reference
Other Information
Depositor Taro Uyeda
Source depositor
Use Condition A
DSC
Remarks
Images
Sequence
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