Strain - Detail

NBRP ID S00476
Strain Name AX2-N2-gfp, vat-gfp, ax-2-vat-gfp
Strain Summary AX2 cells expressing VatM-GFP
Strain Descriptor [act15]:vatM:GFP
Systematic Name DBS0235537
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX2
Mutagenesis Method extrachromosomal_plasmid
Mutagenesis Detail exogenous mutation, pDXA/VatM-GFP
Associated Gene vatM 
Foreign Gene neoR, gfp
Genotype axeA2, axeB2, axeC2, vatM-GFP, neoR
Phenotype neomycin resistant, GFP marked, overexpressor, axenic
Axenic Growth Y
Viable Spore Formation
References Clarke M, Köhler J, Arana Q, Liu T, Heuser J, Gerisch G.
Dynamics of the vacuolar H(+)-ATPase in the contractile vacuole complex and the endosomal pathway of Dictyostelium cells.
J Cell Sci  (2002)  115(Pt 14)   2893-905  
[PubMed ID = 12082150]  [RRC reference
Gerisch G, Heuser J, Clarke M.
Tubular-vesicular transformation in the contractile vacuole system of Dictyostelium.
Cell Biol Int  (2002)  26(10)   845-52  
[PubMed ID = 12421575]  [RRC reference
Other Information
Depositor DSC
Source M.Clarke
Use Condition A
DSC DBS0235537