Strain - Detail

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NBRP ID S00483
Strain Name AX4tgrB1-tgrC1-, tgrB1-/tgrC1-
Strain Summary tgrB1/tgrC1 double null strain with the pyr56 gene inserted in the tgrB1-C1 locus; the pyr56 gene is deleted from native locus and inserted in tgrB1 and tgrC1 locus
Strain Descriptor tgrB1-/tgrC1-
Systematic Name AK1540
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX4/ura- (AK1539)
Mutagenesis Method homologous recombination
Mutagenesis Detail endogenous deletion with insertion, tgrB1/tgrC1-pyr56+
Associated Gene tgrB1  tgrC1 
Foreign Gene
Genotype
Phenotype axenic
Axenic Growth Y
Viable Spore Formation NO
References Hirose S, Benabentos R, Ho HI, Kuspa A, Shaulsky G.
Self-recognition in social amoebae is mediated by allelic pairs of tiger genes.
Science  (2011)  333(6041)   467-70  
[PubMed ID = 21700835]  [RRC reference
Other Information
Depositor DSC
Source R. Benabentos
Use Condition A
DSC DBS0349731
Remarks Rarely small fruit bodies are formed. it is difficult.
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