Strain - Detail

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NBRP ID S90067
Strain Name MCF1
Strain Summary REMI mutant defective in macrocyst formation
Strain Descriptor (macA)
Systematic Name MCF1
Species Dictyostelium discoideum
Category Recombinant
Parental Strain KAX3
Mutagenesis Method REMI
Mutagenesis Detail pUCBsrDBam
Associated Gene macA 
Foreign Gene bsR
Genotype axeA2, axeB2, axeC2, macA-, bsr
Phenotype axenic, blasticidin resistance, no macrocysts, no cell fusion
Axenic Growth Y
Viable Spore Formation OK
References Araki Y, Shimizu HD, Saeki K, Okamoto M, Yamada L, Ishida K, Sawada H, Urushihara H.
A surface glycoprotein indispensable for gamete fusion in the social amoeba Dictyostelium discoideum.
Eukaryotic Cell  (2012)  11(5)   638-44  
[PubMed ID = 22389384]  [RRC reference
Other Information
Depositor Hideko Urushihara
Source H.D. Shimizu
Use Condition A
DSC
Remarks
Images
Sequence
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