Strain - Detail

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NBRP ID S90100
Strain Name RacF2-CA05-1
Strain Summary overexpressor of constitutively-active RacF2 in racF2-(V12); resistant to blasticidin and neomycin; axenic
Strain Descriptor V12/[act15]:gfp:racF2(G12V)
Systematic Name racF2(G12V)
Species Dictyostelium discoideum
Category Recombinant
Parental Strain racF2-(V12)
Mutagenesis Method random Insertion
Mutagenesis Detail pGFP:RacF2(G12V)
Associated Gene racF2 
Foreign Gene amp, bsR, neoR, gfp
Genotype racF2-/[pRacF2-CA], neoR, gfp
Phenotype
Axenic Growth N
Viable Spore Formation OK
References Muramoto T, Urushihara H.
Small GTPase RacF2 affects sexual cell fusion and asexual development in Dictyostelium discoideum through the regulation of cell adhesion.
Dev. Growth Differ.  (2006)  48(3)   199-208  
[PubMed ID = 16573737]  [RRC reference
Other Information racF2=GEG02
Depositor Hideko Urushihara
Source T. Muramoto
Use Condition A
DSC
Remarks
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Sequence
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