Strain - Detail

NBRP ID S90253
Strain Name MO2930, HM2930-2
Strain Summary matA null overexpressing matS in AX2
Strain Descriptor matA-,[act15]matS/AX2
Systematic Name MO2930
Species Dictyostelium discoideum
Category Recombinant
Parental Strain HM1524
Mutagenesis Method random insertion
Mutagenesis Detail
Associated Gene matA  matS 
Foreign Gene neoR
Genotype axeA2,axeB2,axeC2,neoR,matA-,matS[OE]
Phenotype mating-type conversion to type III
Axenic Growth Y
Viable Spore Formation OK
References Okamoto M, Yamada L, Fujisaki Y, Bloomfield G, Yoshida K, Kuwayama H, Sawada H, Mori T, Urushihara H.
Two HAP2-GCS1 homologs responsible for gamete interactions in the cellular slime mold with multiple mating types: Implication for common mechanisms of sexual reproduction shared by plants and protozoa and for male-female differentiation.
Dev Biol  (2016)  415(1)   6-13  
[PubMed ID = 27189178]  [RRC reference
Other Information original HM2930 lost its ability of macrocyst formation
Depositor Hideko Urushihara
Source M. Okamoto
Use Condition A