Strain - Detail

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NBRP ID S90277
Strain Name arpB+/arpB-
Strain Summary Arp2 heterozygous diploid arpB-/arpB+. (bsR cassette inserted in one copy of arpB)
Strain Descriptor arpB+/arpB-
Systematic Name DJK45
Species Dictyostelium discoideum
Category Recombinant
Parental Strain DIR1 (DBS0235911)
Mutagenesis Method homologous recombination
Mutagenesis Detail endogenous deletion, pRHI76
Associated Gene arpB 
Foreign Gene bsR
Genotype axeA1,axeB1,axeC1,pyr5-6-[pRG24],pyr5-6-[PYR5-6-SalI-linker],thy-[PYR5-6-THY1],ura+,thy+, arpB+/-[mycDdarpBpRHI76], bsR
Phenotype blasticidin resistant, axenic, mutant, heterozygous diploid
Axenic Growth Y
Viable Spore Formation OK
References Zaki M, King J, F├╝tterer K, Insall RH.
Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics.
BMC Genet  (2007)  8   28  
[PubMed ID = 17553170]  [RRC reference
Other Information
Depositor DSC
Source R. Insall
Use Condition A
DSC DBS0238408
Remarks
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Sequence
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