Strain - Detail

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NBRP ID S90278
Strain Name arpB-/[act15]:myc:arpB
Strain Summary arpB- mutant rescued with myc-tagged Dictyostelium arpB under the control of the act15 promoter.
Strain Descriptor arpB-/[act15]:myc:arpB
Systematic Name MZ10
Species Dictyostelium discoideum
Category Recombinant
Parental Strain DMZ10 (DJK45+pJSK19)
Mutagenesis Method haploid segregant
Mutagenesis Detail
Associated Gene arpB 
Foreign Gene bsR, neoR, myc
Genotype axeA1, axeB1, axeC1, pyr5-6-[pRG24], pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy-, arpB-, bsR, neoR, [act15/myc:arpB]
Phenotype neomycin resistant, Myc-tagged, blasticidin resistant, axenic, mutant, rescued
Axenic Growth Y
Viable Spore Formation OK
References Zaki M, King J, F├╝tterer K, Insall RH.
Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics.
BMC Genet  (2007)  8   28  
[PubMed ID = 17553170]  [RRC reference
Choi CH, Thomason PA, Zaki M, Insall RH, Barber DL.
Phosphorylation of actin-related protein 2 (Arp2) is required for normal development and cAMP chemotaxis in Dictyostelium.
J Biol Chem  (2013)  288(4)   2464-74  
[PubMed ID = 23223240]  [RRC reference
Other Information
Depositor DSC
Source R. Insall
Use Condition A
DSC DBS0238410
Remarks
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