Strain - Detail

NBRP ID S90279
Strain Name sgkA-, sgkAΔ, SA512
Strain Summary sgkA null mutant. The SalI-XbaI fragment containing the sgkA gene from cDNA clone SLG-787 was cloned into pBluescript. The EcoRI site within the sgkA gene was replaced with a BamHI site into which the 1.4-kb bsr cassette was cloned. Ax4 cells were electroporated with the above vector, linearized with SalI, and selected for blasticidin resistance. The resulting sgkAΔ strain is SA576.
Strain Descriptor
Systematic Name SA576
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX4 (DBS0235549)
Mutagenesis Method homologous recombination
Mutagenesis Detail
Associated Gene sgkA 
Foreign Gene bsR
Genotype axeA1, axeB1, axeC1,bsR, sgkA-
Phenotype axenic, blasticidin resistant,null mutant
Axenic Growth Y
Viable Spore Formation OK
References Min J, Traynor D, Stegner AL, Zhang L, Hanigan MH, Alexander H, Alexander S.
Sphingosine kinase regulates the sensitivity of Dictyostelium discoideum cells to the anticancer drug cisplatin.
Eukaryot Cell  (2005)  4(1)   178-89  
[PubMed ID = 15643073]  [RRC reference
Other Information
Depositor DSC
Source S. Alexander
Use Condition A
DSC DBS0236931