Strain - Detail

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NBRP ID S90282
Strain Name mfeA-
Strain Summary mfeA null mutant (peroxisomal multifunctional enzyme; MFE1))
Strain Descriptor mfeA-
Systematic Name MFE1 null
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX2
Mutagenesis Method homologous recombination
Mutagenesis Detail mfeA-KO
Associated Gene mfeA 
Foreign Gene bsR
Genotype axeA2,axeB2,axeC2,bsR,mfeA-[mfeA-KO]
Phenotype axenic,blasticidin resistant, null mutant
Axenic Growth Y
Viable Spore Formation OK
References Matsuoka S, Saito T, Kuwayama H, Morita N, Ochiai H, Maeda M.
MFE1, a member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family, affects fatty acid metabolism necessary for morphogenesis in Dictyostelium spp.
Eukaryotic Cell  (2003)  2(3)   638-45  
[PubMed ID = 12796309]  [RRC reference
Matsuoka S, Kuwayama H, Ikeno D, Oyama M, Maeda M.
Defect in peroxisomal multifunctional enzyme MFE1 affects cAMP relay in Dictyostelium.
Dev. Growth Differ.  (2004)  46(2)   195-9  
[PubMed ID = 15066197]  [RRC reference
Other Information
Depositor Mineko Maeda
Source DSC
Use Condition A
DSC DBS0236544