Strain - Detail

NBRP ID S90283
Strain Name gefB-ko, Rasgef B-null, RasgefB-
Strain Summary RasgefB-null mutant. Does not grow in liquid: trying to do so will suppress the phenotype!
Strain Descriptor gefB-
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX3
Mutagenesis Method homologous recombination
Mutagenesis Detail endogenous mutation, gefB-bsr
Associated Gene gefB 
Foreign Gene bsR
Genotype axeA1,axeB1,axeC1,bsR,gefB-[gefB-bsr]
Phenotype axenic,drug resistant, null mutant
Axenic Growth N
Viable Spore Formation OK
References Wilkins A, Chubb JR, Insall RH.
A novel Dictyostelium RasGEF is required for normal endocytosis, cell motility and multicellular development.
Curr Biol  (2000)  10(22)   1427-37  
[PubMed ID = 11102804]  [RRC reference
Wilkins A, Szafranski K, Fraser DJ, Bakthavatsalam D, Müller R, Fisher PR, Glöckner G, Eichinger L, Noegel AA, Insall RH.
The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles.
Genome Biol  (2005)  6(8)   R68  
[PubMed ID = 16086850]  [RRC reference
Other Information
Depositor DSC
Source R. Insall
Use Condition A
DSC DBS0236009