Strain - Detail

NBRP ID S90288
Strain Name [RNAi]trap1
Strain Summary Dd-TRAP1-knockdown cells (RNAi from tetracyline-regulated promoter); neomycin resistant; blasticidin resistant
Strain Descriptor
Systematic Name TRAP-1, Dd-TRAP1-KD, Trap-Kd, Dd-TRAP1-knockdown, RNAi, TRP1 RNAi cells
Species Dictyostelium discoideum
Category Recombinant
Parental Strain MB35
Mutagenesis Method knockdown
Mutagenesis Detail exogenous mutation, TRAP1-RNAi MB38
Associated Gene trap1 
Foreign Gene bsR, neoR
Genotype axeA2,axeB2,axeC2,[MB35]neoR,[TRAP1-RNAi MB38],bsR
Phenotype drug resistant, axenic, mutant
Axenic Growth Y
Viable Spore Formation OK
References Morita T, Amagai A, Maeda Y.
Unique behavior of a dictyostelium homologue of TRAP-1, coupling with differentiation of D. discoideum cells.
Exp Cell Res  (2002)  280(1)   45-54  
[PubMed ID = 12372338]  [RRC reference
Morita T, Amagai A, Maeda Y.
Translocation of the Dictyostelium TRAP1 homologue to mitochondria induces a novel prestarvation response.
J Cell Sci  (2004)  117(Pt 24)   5759-70  
[PubMed ID = 15507488]  [RRC reference
Morita T, Yamaguchi H, Amagai A, Maeda Y.
Involvement of the TRAP-1 homologue, Dd-TRAP1, in spore differentiation during Dictyostelium development.
Exp Cell Res  (2005)  303(2)   425-31  
[PubMed ID = 15652354]  [RRC reference
Blaauw M, Linskens MH, van Haastert PJ.
Efficient control of gene expression by a tetracycline-dependent transactivator in single Dictyostelium discoideum cells.
Gene  (2000)  252(1-2)   71-82  
[PubMed ID = 10903439]  [RRC reference
Other Information
Depositor Yasuo Maeda
Source DSC
Use Condition A
DSC DBS0237315