Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S90288 | |
| Strain Name | [RNAi]trap1 | |
| Strain Summary | Dd-TRAP1-knockdown cells (RNAi from tetracyline-regulated promoter); neomycin resistant; blasticidin resistant | |
| Strain Descriptor | ||
| Systematic Name | TRAP-1, Dd-TRAP1-KD, Trap-Kd, Dd-TRAP1-knockdown, RNAi, TRP1 RNAi cells | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | MB35 | |
| Mutagenesis Method | knockdown | |
| Mutagenesis Detail | exogenous mutation, TRAP1-RNAi MB38 | |
| Associated Gene | trap1 | |
| Foreign Gene | bsR, neoR | |
| Genotype | axeA2,axeB2,axeC2,[MB35]neoR,[TRAP1-RNAi MB38],bsR | |
| Phenotype | drug resistant, axenic, mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Morita T, Amagai A, Maeda Y.
Unique behavior of a dictyostelium homologue of TRAP-1, coupling with differentiation of D. discoideum cells. Exp Cell Res (2002) 280(1) 45-54 [PubMed ID = 12372338] [RRC reference] Morita T, Amagai A, Maeda Y. Translocation of the Dictyostelium TRAP1 homologue to mitochondria induces a novel prestarvation response. J Cell Sci (2004) 117(Pt 24) 5759-70 [PubMed ID = 15507488] [RRC reference] Morita T, Yamaguchi H, Amagai A, Maeda Y. Involvement of the TRAP-1 homologue, Dd-TRAP1, in spore differentiation during Dictyostelium development. Exp Cell Res (2005) 303(2) 425-31 [PubMed ID = 15652354] [RRC reference] Blaauw M, Linskens MH, van Haastert PJ. Efficient control of gene expression by a tetracycline-dependent transactivator in single Dictyostelium discoideum cells. Gene (2000) 252(1-2) 71-82 [PubMed ID = 10903439] [RRC reference] |
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| Other Information | ||
| Depositor | Yasuo Maeda | |
| Position | ||
| Source | DSC | |
| Use Condition | A | |
| DSC | DBS0237315 | |
| Remarks | ||
| Images | ||
| Sequence | ||
