Strain - Detail

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NBRP ID S90293
Strain Name DIA2-AS
Strain Summary dia2 antisense mutant
Strain Descriptor [act15]:[AS]dia2
Systematic Name DIA2-AS
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX2 (DBS0235531)
Mutagenesis Method antisense
Mutagenesis Detail exogenous mutation, pAct15-DIA2AS
Associated Gene dia2 
Foreign Gene neoR
Genotype axeA2, axeB2, axeC2, neoR, [act15]:[AS]dia2
Phenotype neomycin resistant, underexpressor, axenic, mutant
Axenic Growth Y
Viable Spore Formation NO
References Hirata K, Amagai A, Chae SC, Hirose S, Maeda Y.
Involvements of a novel protein, DIA2, in cAMP signaling and spore differentiation during Dictyostelium development.
Differentiation  (2008)  76(3)   310-22  
[PubMed ID = 17825085]  [RRC reference
Chae SC, Inazu Y, Amagai A, Maeda Y.
Underexpression of a novel gene, dia2, impairs the transition of Dictyostelium cells from growth to differentiation.
Biochem Biophys Res Commun  (1998)  252(1)   278-83  
[PubMed ID = 9813183]  [RRC reference
Other Information
Depositor DSC
Source A. Amagai
Use Condition A
DSC DBS0237227
Remarks
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