Strain - Detail

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NBRP ID S90308
Strain Name HPS400
Strain Summary thymidine auxotroph (tester strain) (strain is not thy-)
Strain Descriptor HPS400
Systematic Name HPS400
Species Dictyostelium discoideum
Category Recombinant
Parental Strain HPS83
Mutagenesis Method chemical
Mutagenesis Detail MNNG
Associated Gene
Foreign Gene
Genotype axeA1, axeB1, axeC1, cycA1, tsgA1, acrD369, ebrB364, sprH351, manA1, tmpA600, thy-
Phenotype cycloheximide resistance, temperature conditional mutant, multiple mutant, axenic, thymidine auxotroph, mutant
Axenic Growth Y
Viable Spore Formation OK
References Shizukuishi S, Taniguchi T, Shibata S, Nakamura R, Tsunemitsu A, Uesugi Y.
Purification and some properties of alpha-L-fucosidase isolated from Streptococcus sanguis.
J. Dent. Res.  (1978)  57(11-12)   1028-35  
[PubMed ID = 281337]  [RRC reference
Podgorski G, Deering RA.
Thymidine-requiring mutants of Dictyostelium discoideum.
Mol. Cell. Biol.  (1984)  4(12)   2784-91  
[PubMed ID = 6527688]  [RRC reference
Other Information
Depositor DSC
Source K. Saxe
Use Condition A
DSC DBS0236313