Strain - Detail

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NBRP ID S90420
Strain Name AX2/chcA-, chc-, 5E2, DdCHC-KO, chcA-minus, chcA-
Strain Summary Clathrin heavy chain (chcA) null mutant in AX2
Strain Descriptor chcA-
Systematic Name HO103
Species Dictyostelium discoideum
Category Recombinant
Parental Strain AX2 (DBS0237699)
Mutagenesis Method homologous recombination
Mutagenesis Detail endogenous deletion, pChcA-bsr
Associated Gene chcA 
Foreign Gene bsR
Genotype axeA2, axeB2, axeC2, chcA-[pChcA-bsr], bsR
Phenotype axenic, null mutant, blasticidin resistant
Axenic Growth Y
Viable Spore Formation NO
References Niswonger ML, O'Halloran TJ.
Clathrin heavy chain is required for spore cell but not stalk cell differentiation in Dictyostelium discoideum.
Development  (1997)  124(2)   443-51  
[PubMed ID = 9053320]  [RRC reference
Other Information abolished growth - Environment: in suspension
Depositor DSC
Source T. OHalloran
Use Condition A
DSC DBS0235587