Strain - Detail

NBRP ID S90427
Strain Name HPN2-III, 73C7
Strain Summary hapA-KO in MO2930, clone 3
Strain Descriptor hapA-/matA-/matS[OE]
Systematic Name 73C7
Species Dictyostelium discoideum
Category Recombinant
Parental Strain MO2930
Mutagenesis Method homologous recombination
Mutagenesis Detail pCR4[hapA-]
Associated Gene hgrA (hapA) 
Foreign Gene bsR, neoR
Genotype axeA2,axeB2,axeC2,matA-,matS[OE],hapA-,neoR,bsR
Phenotype normal
Axenic Growth Y
Viable Spore Formation OK
References Okamoto M, Yamada L, Fujisaki Y, Bloomfield G, Yoshida K, Kuwayama H, Sawada H, Mori T, Urushihara H.
Two HAP2-GCS1 homologs responsible for gamete interactions in the cellular slime mold with multiple mating types: Implication for common mechanisms of sexual reproduction shared by plants and protozoa and for male-female differentiation.
Dev Biol  (2016)  415(1)   6-13  
[PubMed ID = 27189178]  [RRC reference
Other Information type-III congenic
Depositor Hideko Urushihara
Source Y. Fujisaki
Use Condition A
DSC none