Picked up papers related to NBRP cellular slime mold resources
CRISPR/Cas9-based genome-wide screening of Dictyostelium
Takanori Ogasawara , Jun Watanabe, Remi Adachi, Yusuke Ono, Yoichiro Kamimura, Tetsuya Muramoto.
Sci Rep. 2022 Jul 2;12(1):11215. doi: 10.1038/s41598-022-15500-3.
Genome-wide screening is a powerful method used to identify genes and pathways associated with a phenotype of interest. In this paper, a genome-wide screening method for gene-edited strains has been developed using CRISPR/Cas9 to target all genes in the cellular slime mold genome. Resources related to this paper are archived and available at NBRP Cellular Slime molds.
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The social amoebae, cellular slime molds, are nonpathogenic eukaryotic microorganisms and known
for their unique life cycles shuttling between unicellular and multicellular stages.
Since the amoebae are easily amenable to molecular biological manipulations, they serve as
useful model organisms for both basic and applied sciences.
As NBRP Resource Center, Faculty of Life and Environmental Sciences, University of Tsukuba,
takes care of the biological resources of the social amoebae in Japan,
and distribute them upon request from domestic and oversea users.
Cellular slime mold strains are being collected mainly from Japanese laboratories.
Cellular slime molds vectors and plasmids are available. In addition, Dictyostelium discoideum cDNA clones covering the entire coding sequences are being collected.
