Plasmid Vector - Detail
| Request |
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|---|---|---|
| NBRP ID | G90426 | |
| Name | pTM1285 | |
| Description | All-in-one CRISPR/Cas9 vector Cas9-NLS-GFP, tRNA-sgRNA (BpiI) Transient expression (Neo) | |
| Accession | ||
| DSC-ID | ||
| Associated Gene(s) | SpCas9 | |
| Backbone Plasmid | pBluescript II | |
| Antibiotic Resistance | ampr, neor | |
| References |
Sekine R, Kawata T, Muramoto T.
CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium. Sci Rep (2018) 8(1) 8471 [PubMed ID = 29855514] [RRC reference] Ogasawara T, Watanabe J, Adachi R, Ono Y, Kamimura Y, Muramoto T. CRISPR/Cas9-based genome-wide screening of Dictyostelium. Sci Rep (2022) 12(1) 11215 [PubMed ID = 35780186] [RRC reference] Günther M, Reimer C, Herbst R, Kufs JE, Rautschek J, Ueberschaar N, Zhang S, Peschel G, Reimer L, Regestein L, Valiante V, Hillmann F, Stallforth P. Yellow polyketide pigment suppresses premature hatching in social amoeba. Proc Natl Acad Sci U S A (2022) 119(43) e2116122119 [PubMed ID = 36252029] [RRC reference] Nguyen LTS, Robinson DN. The lectin Discoidin I acts in the cytoplasm to help assemble the contractile machinery. J Cell Biol (2022) 221(11) [PubMed ID = 36165849] [RRC reference] Wang Y, Papayova M, Warren E, Pears CJ. mTORC1 pathway activity biases cell fate choice. Sci Rep (2024) 14(1) 20832 [PubMed ID = 39242621] [RRC reference] Aoki MM, Kisiala AB, Mathavarajah S, Schincaglia A, Treverton J, Habib E, Dellaire G, Emery RJN, Brunetti CR, Huber RJ. From biosynthesis and beyond-Loss or overexpression of the cytokinin synthesis gene, iptA, alters cytokinesis and mitochondrial and amino acid metabolism in Dictyostelium discoideum. FASEB J (2024) 38(1) e23366 [PubMed ID = 38102957] [RRC reference] |
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| Map | 1285.pdf | |
| Sequence | pTM1285.txt | |
| Depositor | Tetsuya Muramoto | |
| Position | ||
| Source | Depositor | |
| Other Information | Ideal for gene knockout | |
| Use Condition | B1 | |
