Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00007 | |
| Strain Name | GMP1, Ddpi3k1-/2-, Ddpi3k1/2-null, ΔDdpik1 ΔDdpik2,pi3k1-/2-, pi3k1-2- | |
| Strain Summary | pikA/pikB double null mutant | |
| Strain Descriptor | pikA-/pikB- | |
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | KAX3 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | ||
| Associated Gene | pikA pikB | |
| Foreign Gene | bsR | |
| Genotype | axeA1,axeB1,axeC1,bsR,pikB-,pikA- | |
| Phenotype | axenic, blasticidin resistant, drug resistant, multiple null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | NO | |
| References |
Zhou K, Takegawa K, Emr SD, Firtel RA.
A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development. Mol Cell Biol (1995) 15(10) 5645-56 [PubMed ID = 7565716] [RRC reference] Meili R, Ellsworth C, Firtel RA. A novel Akt/PKB-related kinase is essential for morphogenesis in Dictyostelium. Curr Biol (2000) 10(12) 708-17 [PubMed ID = 10873800] [RRC reference] Funamoto S, Milan K, Meili R, Firtel RA. Role of phosphatidylinositol 3' kinase and a downstream pleckstrin homology domain-containing protein in controlling chemotaxis in dictyostelium. J Cell Biol (2001) 153(4) 795-810 [PubMed ID = 11352940] [RRC reference] Chung CY, Potikyan G, Firtel RA. Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKa. Mol Cell (2001) 7(5) 937-47 [PubMed ID = 11389841] [RRC reference] Funamoto S, Meili R, Lee S, Parry L, Firtel RA. Spatial and temporal regulation of 3-phosphoinositides by PI 3-kinase and PTEN mediates chemotaxis. Cell (2002) 109(5) 611-23 [PubMed ID = 12062104] [RRC reference] |
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| Other Information | originally deposited by Rick Firtel to Dicty Stock Center | |
| Depositor | DSC | |
| Position | ||
| Source | R. Firtel | |
| Use Condition | A | |
| DSC | DBS0236766 | |
| Remarks | ||
| Images | ||
| Sequence | ||
