Strain - Detail

NBRP ID S00007
Strain Name GMP1, Ddpi3k1-/2-, Ddpi3k1/2-null, ΔDdpik1 ΔDdpik2,pi3k1-/2-, pi3k1-2-
Strain Summary pikA/pikB double null mutant
Strain Descriptor pikA-/pikB-
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain KAX3
Mutagenesis Method homologous recombination
Mutagenesis Detail
Associated Gene pikA  pikB 
Foreign Gene bsR
Genotype axeA1,axeB1,axeC1,bsR,pikB-,pikA-
Phenotype axenic, blasticidin resistant, drug resistant, multiple null mutant
Axenic Growth Y
Viable Spore Formation NO
References Zhou K, Takegawa K, Emr SD, Firtel RA.
A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development.
Mol Cell Biol  (1995)  15(10)   5645-56  
[PubMed ID = 7565716]  [RRC reference
Meili R, Ellsworth C, Firtel RA.
A novel Akt/PKB-related kinase is essential for morphogenesis in Dictyostelium.
Curr Biol  (2000)  10(12)   708-17  
[PubMed ID = 10873800]  [RRC reference
Funamoto S, Milan K, Meili R, Firtel RA.
Role of phosphatidylinositol 3' kinase and a downstream pleckstrin homology domain-containing protein in controlling chemotaxis in dictyostelium.
J Cell Biol  (2001)  153(4)   795-810  
[PubMed ID = 11352940]  [RRC reference
Chung CY, Potikyan G, Firtel RA.
Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKa.
Mol Cell  (2001)  7(5)   937-47  
[PubMed ID = 11389841]  [RRC reference
Funamoto S, Meili R, Lee S, Parry L, Firtel RA.
Spatial and temporal regulation of 3-phosphoinositides by PI 3-kinase and PTEN mediates chemotaxis.
Cell  (2002)  109(5)   611-23  
[PubMed ID = 12062104]  [RRC reference
Other Information originally deposited by Rick Firtel to Dicty Stock Center
Depositor DSC
Source R. Firtel
Use Condition A
DSC DBS0236766