Notice: Suspension of Services Due to the Network maintenance work of NIG

The services on our website will be stopped during the period of time stated below due to the network maintenance work of NIG. We apologize for the inconvenience and we thank you for your understanding in this matter.

Sat. 6th Jun. 2020 8:30 -17:30

Strain - Detail

NBRP ID S00007
Strain Name GMP1, Ddpi3k1-/2-, Ddpi3k1/2-null, ΔDdpik1 ΔDdpik2,pi3k1-/2-, pi3k1-2-
Strain Summary pikA/pikB double null mutant
Strain Descriptor pikA-/pikB-
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain KAX3
Mutagenesis Method homologous recombination
Mutagenesis Detail
Associated Gene pikA  pikB 
Foreign Gene bsR
Genotype axeA1,axeB1,axeC1,bsR,pikB-,pikA-
Phenotype axenic, blasticidin resistant, drug resistant, multiple null mutant
Axenic Growth Y
Viable Spore Formation NO
References Zhou K, Takegawa K, Emr SD, Firtel RA.
A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development.
Mol. Cell. Biol.  (1995)  15(10)   5645-56  
[PubMed ID = 7565716]  [RRC reference
Meili R, Ellsworth C, Firtel RA.
A novel Akt/PKB-related kinase is essential for morphogenesis in Dictyostelium.
Curr. Biol.  (2000)  10(12)   708-17  
[PubMed ID = 10873800]  [RRC reference
Funamoto S, Milan K, Meili R, Firtel RA.
Role of phosphatidylinositol 3' kinase and a downstream pleckstrin homology domain-containing protein in controlling chemotaxis in dictyostelium.
J. Cell Biol.  (2001)  153(4)   795-810  
[PubMed ID = 11352940]  [RRC reference
Chung CY, Potikyan G, Firtel RA.
Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKa.
Mol. Cell  (2001)  7(5)   937-47  
[PubMed ID = 11389841]  [RRC reference
Funamoto S, Meili R, Lee S, Parry L, Firtel RA.
Spatial and temporal regulation of 3-phosphoinositides by PI 3-kinase and PTEN mediates chemotaxis.
Cell  (2002)  109(5)   611-23  
[PubMed ID = 12062104]  [RRC reference
Other Information originally deposited by Rick Firtel to Dicty Stock Center
Depositor DSC
Source R. Firtel
Use Condition A
DSC DBS0236766