Strain - Detail

NBRP ID S00033
Strain Name RCL-,RLC-null, RMLC-null,rlc null, rmlc null
Strain Summary Mysosin regulatory light chain null mutant
Strain Descriptor mlcR-
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain JH10
Mutagenesis Method homologous recombination
Mutagenesis Detail pRMLC-thy1
Associated Gene
Foreign Gene
Genotype axeA1, axeB1, axeC1, mlcR-[pRMLC-thy1],pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], thy+,ura+,
Phenotype axenic, null mutant
Axenic Growth Y
Viable Spore Formation NO
References Chen P, Ostrow BD, Tafuri SR, Chisholm RL.
Targeted disruption of the Dictyostelium RMLC gene produces cells defective in cytokinesis and development.
J. Cell Biol.  (1994)  127(6 Pt 2)   1933-44  
[PubMed ID = 7806571]  [RRC reference
Ostrow BD, Chen P, Chisholm RL.
Expression of a myosin regulatory light chain phosphorylation site mutant complements the cytokinesis and developmental defects of Dictyostelium RMLC null cells.
J. Cell Biol.  (1994)  127(6 Pt 2)   1945-55  
[PubMed ID = 7806572]  [RRC reference
Other Information Originally deposited by R. Chisholm
Depositor DSC
Source R.Chisholm
Use Condition A
DSC DBS0236567