Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00033 | |
| Strain Name | RCL-,RLC-null, RMLC-null,rlc null, rmlc null | |
| Strain Summary | Mysosin regulatory light chain null mutant | |
| Strain Descriptor | mlcR- | |
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | JH10 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | pRMLC-thy1 | |
| Associated Gene | ||
| Foreign Gene | ||
| Genotype | axeA1, axeB1, axeC1, mlcR-[pRMLC-thy1],pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], thy+,ura+, | |
| Phenotype | axenic, null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | NO | |
| References |
Chen P, Ostrow BD, Tafuri SR, Chisholm RL.
Targeted disruption of the Dictyostelium RMLC gene produces cells defective in cytokinesis and development. J Cell Biol (1994) 127(6 Pt 2) 1933-44 [PubMed ID = 7806571] [RRC reference] Ostrow BD, Chen P, Chisholm RL. Expression of a myosin regulatory light chain phosphorylation site mutant complements the cytokinesis and developmental defects of Dictyostelium RMLC null cells. J Cell Biol (1994) 127(6 Pt 2) 1945-55 [PubMed ID = 7806572] [RRC reference] |
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| Other Information | Originally deposited by R. Chisholm | |
| Depositor | DSC | |
| Position | ||
| Source | R.Chisholm | |
| Use Condition | A | |
| DSC | DBS0236567 | |
| Remarks | ||
| Images | ||
| Sequence | ||
