Strain - Detail

NBRP ID S00033
Strain Name RCL-,RLC-null, RMLC-null,rlc null, rmlc null
Strain Summary Mysosin regulatory light chain null mutant
Strain Descriptor mlcR-
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain JH10
Mutagenesis Method homologous recombination
Mutagenesis Detail pRMLC-thy1
Associated Gene
Foreign Gene
Genotype axeA1, axeB1, axeC1, mlcR-[pRMLC-thy1],pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], thy+,ura+,
Phenotype axenic, null mutant
Axenic Growth Y
Viable Spore Formation NO
References Chen P, Ostrow BD, Tafuri SR, Chisholm RL.
Targeted disruption of the Dictyostelium RMLC gene produces cells defective in cytokinesis and development.
J Cell Biol  (1994)  127(6 Pt 2)   1933-44  
[PubMed ID = 7806571]  [RRC reference
Ostrow BD, Chen P, Chisholm RL.
Expression of a myosin regulatory light chain phosphorylation site mutant complements the cytokinesis and developmental defects of Dictyostelium RMLC null cells.
J Cell Biol  (1994)  127(6 Pt 2)   1945-55  
[PubMed ID = 7806572]  [RRC reference
Other Information Originally deposited by R. Chisholm
Depositor DSC
Source R.Chisholm
Use Condition A
DSC DBS0236567