Strain - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | S00036 | |
| Strain Name | HDT5-1 | |
| Strain Summary | myoA/myoB double null mutant; thymidine prototroph, neomycin resistant | |
| Strain Descriptor | myoA-/myoB | |
| Systematic Name | HDT5 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | myoA- (DBS0236577) | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | endogenous deletion, pDTb9R | |
| Associated Gene | myoA | myoB | | |
| Foreign Gene | neoR | |
| Genotype | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, myoA-[pDTa19R]thy+, myoB-[pDTb9R]neoR | |
| Phenotype | neomycin resistant, axenic, multiple null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Novak KD, Peterson MD, Reedy MC, Titus MA.
Dictyostelium myosin I double mutants exhibit conditional defects in pinocytosis. J Cell Biol (1995) 131(5) 1205-21 [PubMed ID = 8522584] [RRC reference] |
|
| Other Information | ||
| Depositor | Chikako Kitayama | |
| Position | ||
| Source | M. Titus | |
| Use Condition | A | |
| DSC | DBS0236578 | |
| Remarks | ||
| Images | ||
| Sequence | ||
