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Strain - Detail

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NBRP ID S00036
Strain Name HDT5-1
Strain Summary myoA/myoB double null mutant; thymidine prototroph, neomycin resistant
Strain Descriptor myoA-/myoB
Systematic Name HDT5
Species Dictyostelium discoideum
Category Recombinant
Parental Strain myoA- (DBS0236577)
Mutagenesis Method homologous recombination
Mutagenesis Detail endogenous deletion, pDTb9R
Associated Gene myoA | myoB | 
Foreign Gene neoR
Genotype axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, myoA-[pDTa19R]thy+, myoB-[pDTb9R]neoR
Phenotype neomycin resistant, axenic, multiple null mutant
Axenic Growth Y
Viable Spore Formation OK
References Novak KD, Peterson MD, Reedy MC, Titus MA.
Dictyostelium myosin I double mutants exhibit conditional defects in pinocytosis.
J. Cell Biol.  (1995)  131(5)   1205-21  
[PubMed ID = 8522584]  [RRC reference
Other Information
Depositor Chikako Kitayama
Source M. Titus
Use Condition A
DSC DBS0236578
Remarks
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Sequence
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