Strain - Detail

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NBRP ID S00050
Strain Name HS1 (mhcA-)
Strain Summary myosin II-null
Strain Descriptor mhcA-
Systematic Name HS1
Species Dictyostelium discoideum
Category Recombinant
Parental Strain JH10
Mutagenesis Method homologous recombination
Mutagenesis Detail pTZD1THY (5.8-kb XbaI/ScaI fragment)
Associated Gene mhcA 
Foreign Gene
Genotype axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+,mhcA-[pTZD1THY]
Phenotype axenic
Axenic Growth Y
Viable Spore Formation NO
References Ruppel KM, Uyeda TQ, Spudich JA.
Role of highly conserved lysine 130 of myosin motor domain. In vivo and in vitro characterization of site specifically mutated myosin.
J. Biol. Chem.  (1994)  269(29)   18773-80  
[PubMed ID = 8034630]  [RRC reference
Other Information
Depositor Taro Uyeda
Source J. Spudich
Use Condition A