Strain - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | S00050 | |
| Strain Name | HS1 (mhcA-) | |
| Strain Summary | myosin II-null | |
| Strain Descriptor | mhcA- | |
| Systematic Name | HS1 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | JH10 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | pTZD1THY (5.8-kb XbaI/ScaI fragment) | |
| Associated Gene | mhcA | |
| Foreign Gene | ||
| Genotype | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+,mhcA-[pTZD1THY] | |
| Phenotype | axenic | |
| Axenic Growth | Y | |
| Viable Spore Formation | NO | |
| References |
Ruppel KM, Uyeda TQ, Spudich JA.
Role of highly conserved lysine 130 of myosin motor domain. In vivo and in vitro characterization of site specifically mutated myosin. J Biol Chem (1994) 269(29) 18773-80 [PubMed ID = 8034630] [RRC reference] |
|
| Other Information | ||
| Depositor | Taro Uyeda | |
| Position | ||
| Source | J. Spudich | |
| Use Condition | A | |
| DSC | ||
| Remarks | ||
| Images | ||
| Sequence | ||
