Strain - Detail

You cannot order strains and genes at the same time.
Request
NBRP ID S00251
Strain Name rasGKO-IR15(AX2)
Strain Summary rasG null in AX2; resistant to blasticidin; axenic
Strain Descriptor rasG-
Systematic Name IR15
Species Dictyostelium discoideum
Category Recombinant
Parental Strain Ax2
Mutagenesis Method homologous recombination
Mutagenesis Detail a version of pBsr deltaBam marker (Adachi et al., 1994) with BamHI linkers into the singleBglII site of pRASG
Associated Gene rasG 
Foreign Gene bsR
Genotype axeA2, axeB2, axeC2, rasG::bsR
Phenotype
Axenic Growth Y
Viable Spore Formation OK
References Tuxworth RI, Cheetham JL, Machesky LM, Spiegelmann GB, Weeks G, Insall RH.
Dictyostelium RasG is required for normal motility and cytokinesis, but not growth.
J. Cell Biol.  (1997)  138(3)   605-14  
[PubMed ID = 9245789]  [RRC reference
Kamimura Y, Miyanaga Y, Ueda M.
Heterotrimeric G-protein shuttling via Gip1 extends the dynamic range of eukaryotic chemotaxis.
Proc. Natl. Acad. Sci. U.S.A.  (2016)  113(16)   4356-61  
[PubMed ID = 27044073]  [RRC reference
Other Information
Depositor Hideko Urushihara
Source depositor
Use Condition A
DSC
Remarks
Images
Sequence
page-top