Strain - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | S00251 | |
| Strain Name | rasGKO-IR15(AX2) | |
| Strain Summary | rasG null in AX2; resistant to blasticidin; axenic | |
| Strain Descriptor | rasG- | |
| Systematic Name | IR15 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | Ax2 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | a version of pBsr deltaBam marker (Adachi et al., 1994) with BamHI linkers into the singleBglII site of pRASG | |
| Associated Gene | rasG | |
| Foreign Gene | bsR | |
| Genotype | axeA2, axeB2, axeC2, rasG::bsR | |
| Phenotype | ||
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Tuxworth RI, Cheetham JL, Machesky LM, Spiegelmann GB, Weeks G, Insall RH.
Dictyostelium RasG is required for normal motility and cytokinesis, but not growth. J Cell Biol (1997) 138(3) 605-14 [PubMed ID = 9245789] [RRC reference] Kamimura Y, Miyanaga Y, Ueda M. Heterotrimeric G-protein shuttling via Gip1 extends the dynamic range of eukaryotic chemotaxis. Proc Natl Acad Sci U S A (2016) 113(16) 4356-61 [PubMed ID = 27044073] [RRC reference] |
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| Other Information | ||
| Depositor | Hideko Urushihara | |
| Position | ||
| Source | depositor | |
| Use Condition | A | |
| DSC | ||
| Remarks | ||
| Images | ||
| Sequence | ||
