Strain - Detail
| Request |
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|
|---|---|---|
| NBRP ID | S00255 | |
| Strain Name | talA-null | |
| Strain Summary | talinA null mutant; resistant to blasticidin; axenic | |
| Strain Descriptor | ||
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | Ax2 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | pUC-bsr-talin (EcoRI/HindIII fragment) | |
| Associated Gene | talA | |
| Foreign Gene | bsR | |
| Genotype | axeA2, axeB2, axeC2, talA-[talin-bsr], bsR | |
| Phenotype | ||
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Niewöhner J, Weber I, Maniak M, Müller-Taubenberger A, Gerisch G.
Talin-null cells of Dictyostelium are strongly defective in adhesion to particle and substrate surfaces and slightly impaired in cytokinesis. J Cell Biol (1997) 138(2) 349-61 [PubMed ID = 9230077] [RRC reference] Yamazaki SI, Hashimura H, Morimoto YV, Miyanaga Y, Matsuoka S, Kamimura Y, Ueda M. Talin B regulates collective cell migration via PI3K signaling in Dictyostelium discoideum mounds. Biochem Biophys Res Commun (2020) 525(2) 372-377 [PubMed ID = 32098673] [RRC reference] |
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| Other Information | ||
| Depositor | Hideko Urushihara | |
| Position | ||
| Source | depositor | |
| Use Condition | A | |
| DSC | ||
| Remarks | ||
| Images | ||
| Sequence | ||
