Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00337 | |
| Strain Name | gc-1, gc-2, gca-/sgc- | |
| Strain Summary | gca/sgcA double null mutant in DH1(guanylyl cyclases GCA and sGC) | |
| Strain Descriptor | gca-/sgcA- | |
| Systematic Name | DBS0236000 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | DH1 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | ddgca-bsr、psCycKOpyr | |
| Associated Gene | gcA sgcA | |
| Foreign Gene | bsR | |
| Genotype | axeA1, axeB1, axeC1, pyr5-6-[pRG24], sgcA-[psCycKOpyr],ura+,gca-[ddgca-bsr] | |
| Phenotype | axenic,blasticidin resistant, multiple null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Roelofs J, Snippe H, Kleineidam RG, Van Haastert PJ.
Guanylate cyclase in Dictyostelium discoideum with the topology of mammalian adenylate cyclase. Biochem J (2001) 354(Pt 3) 697-706 [PubMed ID = 11237875] [RRC reference] Roelofs J, Meima M, Schaap P, Van Haastert PJ. The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase. EMBO J (2001) 20(16) 4341-8 [PubMed ID = 11500361] [RRC reference] Roelofs J, Van Haastert PJ. Characterization of two unusual guanylyl cyclases from dictyostelium. J Biol Chem (2002) 277(11) 9167-74 [PubMed ID = 11777934] [RRC reference] |
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| Other Information | ||
| Depositor | Satoshi Sawai | |
| Position | ||
| Source | DSC | |
| Use Condition | A | |
| DSC | DBS0236000 | |
| Remarks | ||
| Images | ||
| Sequence | ||
