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Request |
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NBRP ID |
S90693 |
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Strain Name |
PH(AKT)-GFP in gipA- |
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Strain Summary |
PH(AKT)-GFP in gipA- |
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Strain Descriptor |
gipA- cells expressing PH(AKT)-GFP |
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Systematic Name |
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Species |
Dictyostelium discoideum |
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Category |
Recombinant |
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Parental Strain |
gipA- (S90686) |
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Mutagenesis Method |
extrachromosomal plasmid |
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Mutagenesis Detail |
The PH domain of PKBA was inserted into pBIG vector containing GFP tag at the C terminus |
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Associated Gene |
gipA
pkbA
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Foreign Gene |
gfp, bsR, neoR |
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Genotype |
axeA2, axeB2, axeC2, floxedbsR, gipA-[Gip1ΔBSR], PH(AKT)-GFP[pBIG::neoR] |
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Phenotype |
chemotaxis defect, blasticidin resistant, G418 resistant |
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Axenic Growth |
Y |
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Viable Spore Formation |
Y |
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References |
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Other Information |
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Depositor |
Yoichiro Kamimura |
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Position |
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Source |
depositor |
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Use Condition |
B1 |
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DSC |
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Remarks |
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Images |
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Sequence |
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